Can you provide us more info, so we can better assist you?http://forum.osticket.com/d/discussion//please-read-before-requesting-assistance#latestAdditionally can you tell use what osTicket shows for a message to the agents when they try to login, e.g. "wrong password" or "access denied".In case you have a backup of your the database, look at the backup and compare the passwords. But before you do any modification like changing the passwords since they differ in the backup, PLEASE, do a backup of your database and osTicket installation directory.